FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_28_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_28_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436780
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5910.13530839324144878No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5410.12386098264572554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5230.11973991483126517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT957.6342985E-933.152581
CTATACA604.983967E-429.1619954
TCTATAC700.001226482324.9959953
ATAATTA1151.4963662E-624.3439253
TAGTGCC750.00183292823.3295944
GTCTGGG901.9283027E-423.3295921
CTACTGG800.002667005621.8714941
ATATATG850.003790575520.5849361
TAGGCAT1055.460796E-419.99908341
CGTCTAC900.005276617619.44132812
GGTCTGG1107.469277E-419.09003441
TATAATC1501.4950727E-518.6636752
GCGTCAG2104.261892E-818.3303931
TTAGGCA1150.001006965118.26003540
TAGCCTG1150.001007739718.2579445
TGTATAA1953.9424594E-717.94584311
GACTCAT1200.001340703617.4971967
TAACTCC1200.001340703617.4971964
GCTTGAA3800.016.58198570
CAAGGCA1503.157379E-416.3307154
Produced by FastQC (version 0.11.2)