FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_27_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_27_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399447
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7220.18074988671838815No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6150.15396285364516443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG602.6053385E-740.8109133
CCCTATA400.002422583334.985162
GTATATA450.00431142631.1018161
GTTATAT604.987253E-429.1579511
TACACCG657.9757435E-426.9082935
GTAATAA1056.7757173E-726.6586991
GGTAATA700.001228185324.98942
GTGTATA700.001229082224.98627310
GTATGAT851.3095845E-424.69851
TATTATA851.3106986E-424.6954082
TATAGAA851.3106986E-424.6954082
AACGCCC750.001835468523.3234420
TCAATAC901.932988E-423.320523
TAATAGA850.003798565620.576934
CGTGCTC850.003798565620.5769310
ATATTAT1408.250401E-619.9940241
CGCTATC4400.019.09716470
ACGCTAT4750.018.42708869
CCGTGTG950.007225428718.4109369
CCTAACA1000.00970328617.492582
Produced by FastQC (version 0.11.2)