FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_26_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_26_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747424
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12910.17272659159994863No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG11840.1584107548058398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10230.13687010318105922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA657.957872E-426.9224072
GCAGCGT2000.026.254621
CTATAGT750.001832698223.3327544
TATACCC901.9283191E-423.3327545
AGCGTCA2350.022.3398693
GTGTATA2451.8189894E-1221.42517313
GTATTAG1003.9271856E-421.0036951
CTAGTAA1003.9325128E-420.999483
TATATTA850.003790087820.5877235
TATGCCG3050.019.5116357
CTAATAT900.005275930319.443963
GTATAAG2555.4569682E-1119.21263715
GATATAT1501.493064E-518.669951
GTATGCC3200.018.59577856
CCCCTAC1150.00100637918.2640821
TCCTAGA1551.9835166E-518.0640682
GTACAGG1401.8620679E-417.5030781
GTGTAGA1602.6015014E-517.5030781
TAGCCTT1000.00968867717.4995675
CCTATAC1000.00968867717.4995673
Produced by FastQC (version 0.11.2)