FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_24_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_24_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563093
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6530.11596663428598827No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6470.11490109093879697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTC750.0018371323.3218445
CTATATC750.0018371323.3218444
TAATACT1102.9311657E-522.2617634
GTATAAG1454.6060632E-721.7211591
CCTCTAG850.003791405620.5854131
TATATCG850.0037992220.5780985
TATAGTG1107.4954296E-419.081515
TATTAAT1301.0604165E-418.8368762
GGTGTGA1501.5006146E-518.6574766
TATAGGG950.007226645518.4119833
CAAGACC1351.4161516E-418.1392144
CAATACC1351.4161516E-418.1392144
TAGAGTG1000.00971190717.4913855
CGTACCC1200.001344345617.4913853
TTTACAG1653.4050776E-516.9613423
CTCCTAC1250.001762700816.7976971
AGGGGTT1250.00176689716.7917295
TTATCCT2351.5473597E-716.3749124
CTAGAGT1300.00229638516.1458954
CTGGACA1755.63638E-515.9921234
Produced by FastQC (version 0.11.2)