FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_23_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_23_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562524
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC11330.20141362857406972No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9050.16088202458917308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6430.11430623404512519No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT6310.11217299173013062No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG400.002423348834.985383
CTAGTAC952.8306022E-729.4613693
GGGTTAA500.007218871727.990791
AGGGGGG1850.026.49426370
ACCTATA851.3123668E-424.6933657
GCAGCGT750.001835382123.3256571
TAGTACG901.9326339E-423.3235844
AAGGGGG2453.092282E-1120.00587369
TGTAAGG900.00528633619.4363212
GTATAAG900.00528905419.43459315
ATATACT950.00719763618.4248248
TACTTTA950.00722723918.4117187
GTTTTAA1602.6109486E-517.4942441
TTAGGAG1000.00970772817.4926874
TGGGGGG1000.00970772817.4926875
TATAGGT1000.00970772817.4926874
CTATGTC1000.00971269817.4911359
GAATTAG1554.060707E-415.8012511
TCTAGAC1554.0634553E-415.7998483
CTAGGCT1807.166006E-515.5490564
Produced by FastQC (version 0.11.2)