FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_22_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_22_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494596
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15140.3061084198012115No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG8910.18014702909040914No Hit
CTTCTCTTCTGATATCACTTGCTCGCTTTCTGGATATTCCCATTATCACC5050.10210353500634863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA450.004295701631.12649552
CTTAGCG450.004314887331.0981672
GACTGAG808.6973734E-526.2337727
TCTTAAA1001.4011772E-524.4898072
GTATATA750.001831682723.3330631
GCAGCGT952.7785177E-422.1050071
TATTACC952.7861333E-422.0960672
CTAAGGC800.002655817221.88803363
GTCTACC800.002665196821.8747461
TATAGTA800.002671464821.8658985
TATTAGG850.003796897120.5796682
GTCATAT1055.460934E-419.999771
ACTGAGC1055.483316E-419.9876378
ATATAGG900.00527304619.444221
TTAAGAG900.005285395319.4363543
AGGGGGG1501.4925055E-518.6683470
GTGTAAT950.007205414618.420841
TAGGGGT950.007222254318.413394
CATAGGC1752.7926508E-617.9979745
CTGCTAG1000.00967779617.5015729
Produced by FastQC (version 0.11.2)