FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_21_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_21_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326583
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT4210.12891056791076083No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4200.12860436703686354No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3540.10839510935964211No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3320.10165869013390164No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACG400.00241314535.0111156
TGGGCGC400.0024204134.98966610
CTATTAG450.004300532431.1162171
AAGGGGG1103.1610398E-828.6454569
AGGGGGG1103.165769E-828.64106470
TACTACA700.001226785224.9926172
CTATTGA1102.9114979E-522.2763821
TGTTATG800.002665297821.8718922
TTTAGGC800.00266766921.868543
TTAATGT850.003791521520.5821574
ATTCTAA900.005263937619.4476361
TAATATC900.005277942419.4387044
ATAATAC900.005277942419.4387043
CTAAACT900.005277942419.4387044
GGGGATT950.007205733618.41843225
TACTCTG950.00721210418.4156135
ACTGTGT1150.001007911918.2554788
TCCCCGA1150.001007911918.25547813
TGTAGAC1351.4117139E-418.1427882
CAATACC1200.001340935117.4948334
Produced by FastQC (version 0.11.2)