FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_20_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_20_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437758
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7130.16287537863385707No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5820.13295016881473326No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4600.1050808894411981No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG652.098412E-532.3136561
CCCTTAC552.9872122E-431.8240531
TATATCG604.983926E-429.162055
GTGTTAA500.007199081628.0051631
CTATACT700.001226472324.9960424
ATGGGGT750.001832913123.329644
TAATAGT952.7805715E-422.1017634
GCTCTAG800.002661680821.87903640
GGGGTTT850.00378552920.5896830
ATCACGC850.003793054720.5826247
TACCTTG950.007210229518.4181356
TAAATGC950.007210229518.4181354
TTTGGGG1351.4116231E-418.1452753
TAACCAA1200.001337614517.5032352
GCAGCGT1200.001337614517.503231
TAATGTG1000.00968990217.4972295
TCTATAC1000.00968990217.4972293
ACCTTAT1401.8662341E-417.4952328
GAATAAC1452.431556E-416.8996681
TTATGTA1250.001762106516.797344
Produced by FastQC (version 0.11.2)