FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_19_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_19_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305405
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6680.21872595406100098No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4580.14996480083823122No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC252.3905389E-455.9891243
CCATATA450.00431110231.0999722
CTATGCA450.00432148331.0846929
TGTATCG500.007185993328.0129241
GTATATA500.007214805627.9899751
GTATAAT500.007214805627.9899751
TATGGAT657.9548964E-426.9178453
TGTGCGA657.993057E-426.89581110
ATAATGC800.00266570821.870753
ATTGTAG800.00266570821.870753
AGGGGGG850.003774375220.59773670
GTGTATA850.003806766620.56738513
AGGCATA900.005249144519.45660654
AGTACTG900.00527408419.4406665
ATATAGA900.005279083319.4374831
CGGACAT900.00528408719.434323
TTGGACT950.00720684818.4174734
TACATTG950.00720684818.4174735
GTTCACA950.00721366518.4144551
GGGTGCG950.007234148718.405417
Produced by FastQC (version 0.11.2)