FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_18_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_18_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296532
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6320.21313045472326764No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4910.1655807804891209No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAG400.002416119235.00126633
TACACGG450.004306148731.1069895
GTATAAG604.979343E-429.1628021
ATCGGTA500.00719467828.00573537
TGTAGTG500.007206538727.996295
ATTACAG851.3069969E-424.7026083
ACTGTCG901.9259089E-423.3302428
TAGGCAT1154.1051215E-521.30511747
TATTACA850.003787137620.5855062
CTGTCGA900.00527185519.4418689
TAACTCT900.00527185519.4418684
TTAGGCA950.00719679918.42171946
AGGCATA1351.4075794E-418.14880448
GGAACAG1953.9307452E-717.946345
CGCACTC1000.0096625217.50358639
ATGCAAG1000.00968130917.497681
TACTTTG1250.001759993716.7977737
CCAGTAC1755.6046792E-515.9978813
GTACAAG1350.00294230915.5534941
GTACTTT1350.00294230915.5534946
Produced by FastQC (version 0.11.2)