FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_16_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_16_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050997
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC16330.15537627605026466No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG15930.15157036604290972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11850.11275008396788955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1251.1013253E-725.1969151
AGGGGGG3850.023.64247370
AAGGGGG4250.022.242169
TACCCCG1154.1305364E-521.296675
GTCTAAG850.00379323920.5857161
GTACTAG1205.7224803E-520.4141671
TAGACAG1951.8333594E-819.7364775
GCAGCGT2456.6211214E-1018.5691551
GTATATG1803.6620931E-617.4978581
TATTAGT1602.6162208E-517.4928632
TATAGTA1401.8713124E-417.4928632
TACTAGG1200.001344635317.4928632
TAAGCCA4700.016.38175446
CTAATAG1554.0658657E-415.8007563
CCTGCGC2001.0000533E-515.7435778
AGCGTCA2906.2482286E-915.6840033
TAGAGCC1807.1719514E-515.5499524
AAAGGGG6250.015.12462868
GTATTAT2352.7739952E-614.8917941
CATAGGC4500.014.78230151
Produced by FastQC (version 0.11.2)