FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_15_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_15_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547885
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC11380.20770782189693093No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9820.17923469341193865No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT6390.1166303147558338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6350.11590023453826989No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG459.212419E-538.876825
TATAAGA755.5981087E-527.98875616
TCTATAC851.310557E-424.6982143
ATAATAC1301.6092599E-724.223253
TATATAG750.001835122423.3260922
TCTAGGG952.7846725E-422.0984023
CCGCGCG800.00267160421.8662179
GTGTATG1154.115607E-521.3035681
AGGGGGG2151.1459633E-1021.16781470
GTATAAC850.0037890720.5874841
GCAGCGT1205.7123012E-520.415921
GTATAAG1055.4763496E-419.99196815
GCGTCAG1257.835489E-519.5939184
ATATTAC900.00528287919.438413
TAGCCAT1107.462948E-419.09369965
GATATAG950.00720742218.420381
ATAGGGC950.007218819618.4153353
TATAAGG1150.001009202218.2552032
AAGGGGG2508.60382E-1018.2043269
ACGTAAT1000.00967594417.50255834
Produced by FastQC (version 0.11.2)