FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_14_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_14_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210509
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2660.12636039314233596No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC2520.11970984613484459No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT2440.11590953355913525No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2120.10070828325629783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAG652.0974707E-532.3041843
TTACCAT552.9871135E-431.8147264
AGACTAT450.004302831431.107736
TTTAACA450.004302831431.107733
GTTATAC450.004302831431.107733
AGTTAGG450.004307838631.1003448
GGCTCCC450.004307838631.10034413
AAGGGGG500.007192666628.00361369
TAGGCAA500.007192666628.00361353
TGTGTAC500.007201018327.9969583
GAGGGTA500.007209377427.99030913
GCACCGC700.001224001824.9972846
GAGTGAG750.001831769123.3252567
ATTCAGG1055.4414716E-420.0025791
GTACTCA900.005258908519.4469551
CTTCTAC950.00718617818.423431
TGTATAA950.007205914718.41467710
GATGTGT1200.001337113317.49816
GTGTATA1000.009684158517.4939429
GAAATGT1350.00293804515.5538656
Produced by FastQC (version 0.11.2)