FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_13_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_13_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332472
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGG305.881185E-446.654032
CTAGTAG350.001256152939.989173
ATCTAGC450.00430980731.1026861
CCTGGGC450.00430980731.1026863
ACCCCAT500.00719675928.00505841
GTCCTAA500.0072126427.9924181
TAGCACA500.0072126427.9924184
ACCGTTC901.9323344E-423.3199968
TAATACA800.002667388621.8690764
AAGACCC1003.9327043E-420.9943145
ATACCAT1205.7139405E-520.4111396
TAATACT1055.465304E-419.9945854
AGGGGGG900.005259049619.45088470
ATATATG900.005277388719.4391781
TAGAGTG900.005286577619.433337
CTATAAA1000.00969141217.4952621
GAACCGT1000.00969141217.4952626
AATACTG1000.00969141217.4952625
CTGGACC1000.00969141217.4952624
GAGTCGG1200.001342135717.4926318
Produced by FastQC (version 0.11.2)