FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_12_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_12_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564108
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9870.1749664957774043No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG8180.14500769356222568No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCTA450.00431249631.1024445
ACCGCGT700.001229678324.9863878
CTTTAAT1154.1151266E-521.304081
TAGCCCT850.003794526720.5824994
CCGCGTG850.003800378220.5770249
ATAGACT1205.7233436E-520.4109764
TATAAGA1257.844785E-519.59106416
CTATAAC900.00527398319.4441991
GTATAAG1301.06084626E-418.83589415
ATATACT950.00721775818.415924
TAGGGAC950.00721775818.415924
TAGTATT950.00721775818.415924
ACCCGTG950.007228841518.4110227
CCCTACT1150.001009037618.2557812
AGCGCAT1150.001010841318.25092710
GTATTGT1351.410963E-418.1479191
TATACTG1551.9864467E-518.0594835
GTATATC1000.00968513517.4997791
CAGCTAA1000.00971484617.4904739
TAGAGCA1250.001764377716.7953194
Produced by FastQC (version 0.11.2)