FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_11_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_11_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344740
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4650.13488426060219294No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4410.1279224923130475No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT3950.11457910309218543No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT601.2133563E-535.0030484
GTGTATA552.9952507E-431.80710613
TTAATAC450.00430248231.1138213
AAGGGGG1207.372182E-826.25228569
AGGGGGG1207.372182E-826.25228570
GTATAAG700.001227301424.99129715
TGTATAA851.3095155E-424.6972814
GTATAGG800.002659806321.880081
GCAGCGT800.002659806321.880081
TAGTCTG800.00266204921.8769055
GTCTAGA850.003780369220.5930161
TCTGAGC850.003783549920.5900293
CCGTGAT850.00379310520.58106813
ACCGTCC850.00379310520.5810688
TCCCCAC850.00379310520.58106819
TATAAGA850.00379310520.58106816
GTATTCG1205.6926358E-520.4214081
TAAGCCA1055.458033E-419.99883846
GTTATGT900.00526244619.4489611
AACGGGG900.00528013819.4376757
Produced by FastQC (version 0.11.2)