FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_10_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_10_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557441
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7160.1284440864593742No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7030.12611200109069837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6560.11768061552702438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG701.9239451E-840.0032351
TATTACG703.4921388E-529.994352
GGCATAG1900.025.7938749
GTGTAAG1052.0369114E-523.3352171
GCATAGG1703.7362042E-922.65295450
CATAGGC1656.759001E-821.21954751
GTATTAT1205.7073714E-520.4183161
GTGTACG1055.4587354E-420.0016161
ATATAAG1257.828457E-519.596313
TATAAGC900.005279268619.4407834
ATAGGCA2002.4256224E-819.25673952
TATTATA1107.479989E-419.0873132
GAGTAAT1150.001006584818.2623441
GTATAAG1351.4137974E-418.14310315
AGGCATA2751.6007107E-1017.8196248
TTAGGCA1602.6037107E-517.49984446
TTAGAGG1000.00969480517.4967044
GTGTATA1452.4416661E-416.89185313
TATACAG1250.001763277216.7968372
CACAGCG1250.001764335916.795337
Produced by FastQC (version 0.11.2)