FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2592_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2592_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398257
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG13350.33521068054045505No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12720.3193917495486583No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT6630.1664754166279563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5320.13358208393072812No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC4280.10746829308712716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCT400.002419605234.993914
ACCTAAT450.00429819831.1213281
TATACTA901.7565071E-731.1056945
TACACCG450.004308781631.1056945
GTCTAGG851.3040093E-424.7139991
TCTAGGG800.002666808421.8711952
CATAGGG850.003782030220.59241157
TTATACT1408.241412E-619.9965214
ACTTACC1257.8204306E-519.5965882
ACGCCTT900.005276235319.441065
TTACCCT900.005276235319.441064
AGGCATA4200.019.1729654
TAGGCAT3700.018.92513353
GTTCTAT1301.0527731E-418.8523451
GTATTAT1301.0527731E-418.8523451
TTGACCG950.00722022518.41322120
CATACCT1150.001007620218.257694
GCATAGG3950.017.72511356
TCTCGCC1000.00967528217.5013533
TTAATCT1401.8642943E-417.4969564
Produced by FastQC (version 0.11.2)