FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2591_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2591_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788588
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC24600.3119499662688248No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG21260.26959578385671606No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12290.15584817420503483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12080.15318518668810582No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC8060.10220799707832226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3400.025.74238670
TTAGGCG1506.363225E-721.00578734
AAGGGGG4450.019.6683469
GTATAAC1651.5944279E-619.087691
CTATACT1651.5963651E-619.085274
GAGGCTT1107.4955303E-419.0828519
GTATATG2254.631147E-918.663521
CCTAATA1702.1253381E-618.5227642
TAATCCG1551.9883644E-518.0591815
CTAGTCT1752.801813E-617.9946844
GCGTAGG1200.001339226817.50260238
CCTATAC2005.1614006E-717.4948313
TTAGGCA3650.017.25955646
GTATATA2852.6739144E-1017.1900841
GTTACAT1653.402118E-516.9646843
AGGCATA6000.016.9159648
GCATAGG5600.016.87643850
GGCATAG5400.016.85221749
TACACTA1906.1340634E-616.574055
GTCTTAA1503.1616204E-416.330581
Produced by FastQC (version 0.11.2)