FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2586_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2586_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394841
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7950.20134687127223364No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6840.1732342892455444No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5270.13347144800058758No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACC200.00694807752.5011424
GCGCCCT400.00241725935.0007647
ACCGACC552.9917626E-431.8148448
GGTTAGA851.3055782E-424.7095471
GGTTCCC851.3078262E-424.70328911
CTATTAG1151.4917423E-624.3514391
TATAGCA750.001832027823.3308852
CTAACCA850.003788726620.5860754
ACTAATA1205.7101322E-520.4145242
TATTAAC1055.4616976E-419.99792
TGGACAG1257.816062E-519.5979445
TAGATAG900.00527404919.4424045
ATTATAC2002.4330802E-819.247983
CTAATAC1150.001007132218.2589533
AGGGGGG2155.5631972E-817.90736670
GTCATAT1401.8596531E-417.5025961
TAAGCCA1803.6453785E-617.5003846
TTAGACA1200.001339897717.4981634
TATATCG1000.00968529317.4981635
GTAATAG1000.00968529317.4981633
Produced by FastQC (version 0.11.2)