FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2576_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2576_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452193
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12530.27709407266366354No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG11060.24458582950200466No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7020.1552434469352688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5960.13180212873706582No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAG450.004302093331.1164721
GTGTTAC853.9467086E-628.8284971
TACCCAG500.00721922527.9893365
CCTGCAC500.00721922527.98933614
GCCTTAA808.649107E-526.2545241
AGGGGGG2850.025.79391970
TTATACT1801.0913936E-1125.2681544
GTATAGG750.001829483423.3373531
GGCATAG2155.456968E-1222.79210349
CACTGTG1403.2933713E-722.4889477
CCTCTAC1102.9145156E-522.2765661
TTGCGCA800.002672281621.8642549
CATAGGC1807.226845E-921.39257451
GCATAGG2151.1459633E-1021.16643750
GCTCTAG850.003783479320.5917841
AGGCATA2453.092282E-1119.9990248
AAGGGGG3700.019.86828869
TAGGCAT2302.8376235E-1019.77945347
TACCATG900.00528701119.434897
TCTACAC1651.5945952E-619.0836413
Produced by FastQC (version 0.11.2)