FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2572_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2572_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283320
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC10860.3833121558661584No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9410.33213327686008753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2960.10447550472963434No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA200.00694887652.4957649
ATCTACG400.002417324834.99717733
TTAGCCG400.002417324834.99717713
GAGCGCG604.9772795E-429.1643149
TTAACCC500.007197974228.0026843
GTCGGCC500.00720418127.99774226
TAGAGCG657.9482066E-426.9209047
CCGATCG808.65281E-526.247889
CCTCGAC750.001830498723.3314516
CATAGGC1403.271398E-722.49818445
CTAATAC952.7724463E-422.1073823
CTCACAC800.00266075621.8770943
GTATTAT800.00266075621.8770941
ATCACAC850.003781719420.5902083
TAGACAG850.003785589720.5865755
AATCTAC850.003785589720.58657532
GCAGCAC850.003785589720.5865758
AGTGTGC850.003785589720.5865758
TAATACA1205.694028E-520.4186234
ATAATAG900.00526432919.446313
Produced by FastQC (version 0.11.2)