FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2567_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2567_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225060
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4330.19239313960721585No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4000.1777303830089754No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT2820.12529992002132764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCA200.006963582252.4641769
CTAATAC501.7107463E-434.983893
GTATAGA1052.015986E-829.9928531
TAACAGG500.00721443827.9871146
TAATACT657.960731E-426.9106854
CCTTATA700.001225189324.9940452
TATTACA750.001831007823.3277782
TTATACT1052.0384408E-523.3225924
AGTGGTC750.001835750423.3174118
GTATATC800.002664229121.8697911
GTATACC800.002664229121.8697911
GAGTGTG800.002671115621.8600737
ACTGTTC1003.9381953E-420.985678
ATTATAG1055.464749E-419.9907933
TGTAATA1257.804982E-519.5953332
TAATAGT900.00527797619.4354934
AAAGGGG1651.5717142E-619.10334870
TATTATG1301.05476254E-418.8416652
GTATTAT950.007202925618.4166661
CTGTAAT950.007202925618.4166661
Produced by FastQC (version 0.11.2)