FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2562_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2562_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820361
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9610.11714355021752619No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT9360.11409611134610251No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA350.001258244539.9826210
GCAGCGT1202.1666801E-623.3345741
CCGGTAC800.002658137821.8868462
GTATACG1601.1849588E-619.6885451
GCGTCAG1501.4988671E-518.6619684
TATTATA4000.018.3703732
GCTAAGG1351.409924E-418.15132750
GTGTACG1000.00968511317.5009291
GTATAGG1602.6047483E-517.5009291
TATAACG1200.001342887917.4955962
CATACCC2005.1596726E-717.4955943
CGTGCGA1200.001343436717.4945322
GGGTATG2651.877197E-917.1707251
CTGCTTG6750.017.11410768
CCACTTA2259.4361894E-817.1036789
GTTATAG1653.3922282E-516.9705981
AGCGTCA1653.4011126E-516.9654253
GGCTAAG1250.001761386216.80089249
GCGTGCG1250.001765702216.79474821
TGTCGGG1906.142798E-616.57174510
Produced by FastQC (version 0.11.2)