FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2559_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2559_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333240
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT5530.16594646501020285No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4500.13503781058696435No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG350.00125258140.0123561
GCCTCGC350.00125628639.98833523
GTAGGAC459.211771E-538.871723
TCGCACG400.002422219134.98454317
CTCTAAG400.002422219134.9845432
CGCACGC450.004313426631.09737418
AGGGGGG1500.030.34726170
CCATAGA703.4940047E-529.9867522
TCCCTAT755.5646058E-528.008651
TCCGGGT500.007197554728.00444432
TATAGTG500.007218687327.9876335
CATAAGC500.007218687327.9876333
CACACAG500.007218687327.9876335
ACCTTAT500.007218687327.9876337
TAAGCCC657.9330744E-426.93139534
ATAGTGT959.403906E-625.7780868
AGGTATA700.001227884824.9889587
ATACTGA700.001227884824.9889586
ATATAAG750.001827024423.3405421
AAGGGGG1703.7143764E-922.65745769
Produced by FastQC (version 0.11.2)