FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2556_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2556_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321419
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9370.2915197919226928No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5910.1838721419704498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3410.10609204807432043No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAC200.006950558652.4941643
CTGAGGT703.485749E-529.9966664
ATGTTAC604.975106E-429.1679611
CCAGTAC500.007206673327.996893
TTACACT906.1627634E-627.2191964
TACACTG959.378467E-625.7866085
CTGTGCA700.001225426324.9972219
CTCTGTA901.9261196E-423.3307382
TTAAAGT1154.11117E-521.3019814
TATACAG1003.928079E-420.9976675
CTAATCT1205.7061716E-520.4143984
TGAACTA1055.458889E-419.9977765
AGGTATA900.005272147719.4422827
GTTCTAG1501.4904366E-518.6674961
TAAAGTT1200.001339218517.4980565
TTGGACA1000.00968183517.4980554
CATACCT1000.00968183517.4980559
TATCACT1452.4345198E-416.8946744
GGCATAG1503.149211E-416.33405955
TTCTATA1300.002287673816.152052
Produced by FastQC (version 0.11.2)