FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2555_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2555_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308715
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6630.21476118750303677No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6240.20212817647344639No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4400.14252627828255834No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT4070.13183680741136647No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAG200.00695557952.4840859
TCCCTAT755.568078E-528.0051211
CTCCGGG500.007212997427.991519
GCACTCA700.001226688424.99242249
CTATAGA1102.9104069E-522.2768021
AAGGGGG1656.723167E-821.22288169
TACCCCA850.003780567320.5920015
TCATAGT850.003780567320.5920014
TTGGGGG850.003780567320.5920014
AGGGGGG1558.6644104E-720.3328970
GTTATGG900.00526272419.4481
GTCTAAT950.00719135518.4244211
TAATAGT1150.001004509518.2642084
TAGAAGC1000.00966460817.50324
CCGATCA1000.00969170117.4946949
GTATAAA1250.00175650216.8030741
CCTCTAT1250.00175650216.8030741
CCATGAT1300.002275502516.1646663
TATACAG1554.0375403E-415.8093435
TATCCCT1350.002936490615.5584015
Produced by FastQC (version 0.11.2)