FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2554_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2554_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332147
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC10040.302275799570672No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9400.28300722270560924No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT5390.16227754578545042No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3350.10085895702806287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA652.106879E-532.2912185
GTATTAA500.00721044327.9941561
ACCCGCC500.007226368427.9815087
ACCGTCC851.3121542E-424.6895688
CTGTACG750.001837346723.3179249
GTGTAGA952.7795593E-422.1006471
CCTTAGA800.002673426621.8605542
ATATACA1859.962605E-920.80024
TACAGTG850.003799687120.5746387
CATATAC1055.479664E-419.9867923
GAACCTT900.005284677719.434536
TTATGTA900.005289281719.4316042
TGCTCAC900.005289281719.43160410
CCCTGTG1702.1178148E-618.52275322
TATACAG950.00722129118.411665
GTAGTGG1150.001007373918.2570571
ATAGGCA1351.402743E-418.15803758
TAGGCAT1752.7735823E-618.00709259
GTATTGT1953.9374027E-717.9449711
AGGGGGG1200.001333465917.50953770
Produced by FastQC (version 0.11.2)