FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2547_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2547_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370604
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7770.2096577478926293No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6140.16567549189970965No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT4590.12385187423773084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3730.10064651218011679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1003.237801E-1035.01627370
AGCGTCA501.7117208E-434.9879233
GTCTAGG500.007202057628.0016731
GTGCGGA500.00722582727.98278411
GTATTAG657.946323E-426.9246861
CGGTAGC700.001227453224.9913736
CCTATAC700.001227453224.9913733
CCACTTA851.3121494E-424.6906939
GCAGCGT750.001830059923.3347281
TAGCATC750.001834377323.3252814
AAGGGGG1703.7180143E-922.6575969
ACACAGT1403.285404E-722.4922356
GGTTACG800.00266284321.8763081
CATACCC1154.1207782E-521.2969973
TAAGCCA2001.0659278E-920.99558646
TACCCCA850.003793565820.5811335
TAGACTA850.003793565820.5811335
CCATACC1408.251069E-619.99312
TATAGTA1055.469671E-419.9930972
CAGGTTA900.005264315819.44823352
Produced by FastQC (version 0.11.2)