FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2544_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2544_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304316
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6400.21030770646301872No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5760.18927693581671684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGG305.8822794E-446.65110
GTAATAC450.00430714331.1057783
TAGGGAG604.98078E-429.1616675
AAGGCCG500.00720819727.9952015
TTGGACA657.95378E-426.918464
GTGTCGG700.001226881224.991619
CTGTCCA901.9287245E-423.32559
ATGGGTG952.7781192E-422.1014755
GTATTAC800.002655177821.8856371
TACCCTC800.002665336721.871255
TAGACAG800.002665336721.871255
ATAGTGT800.002665336721.871256
TATAAGG800.002665336721.871252
GTCGGGA800.002667881321.86765711
TAAAGCA850.003788214620.5847074
TAGTGTG850.00379182420.5813269
AGGGGGG1801.6897866E-719.44750470
CTGGATT1107.468463E-419.0876354
AAGGGGG1902.9840703E-718.4239569
TAATGGT950.007205847718.4178944
Produced by FastQC (version 0.11.2)