FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2542_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2542_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283038
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7550.26674863445897723No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7040.24872985252863572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3430.121185141217787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAACC200.006961647452.4713812
CCCATGC352.0901816E-549.9727448
GCGAAGT552.9973697E-431.80083513
TGCGATA552.9973697E-431.80083512
GCCTCGC703.4862074E-529.99424626
CGCACGC604.985661E-429.15591821
AGTAGGG500.00718322928.01443162
CACTAAC500.007201859727.99957547
GTATTAC657.9615595E-426.9131551
TATACTG959.404928E-625.7754155
TCGCACG700.001226903524.99078820
GCACGCC700.001226903524.99078822
GCTTTAT700.001226903524.9907881
TTATACT1454.6074456E-721.7122944
ATATTAA1656.780647E-821.2043041
TCGCACT1205.683359E-520.42357829
CGCACTC1205.683359E-520.42357830
GCACTCC1205.683359E-520.42357831
ACGCCTC1055.4596394E-419.9961624
CACGCCT1055.466149E-419.99262823
Produced by FastQC (version 0.11.2)