FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2525_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2525_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342110
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9030.2639501914588875No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7660.22390459209026334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3670.10727543772470842No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT3560.10406009762941744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGTG450.004303699431.11195835
TCCGTAC450.004309862431.1028618
CAGCACC500.007212733427.9925774
GGCCAAT500.007212733427.99257722
CCGTACC500.007212733427.9925779
ACGGTTG700.001225643824.99702325
CCTATAC750.001825455623.3442061
GACAATC1102.9216966E-522.266827
TTAGGAT1154.1170522E-521.2986984
CATACTC1003.9328806E-420.9944325
GCATACG900.005255261419.4535051
GACAGTG900.00527752519.439297
CTGGTCA900.00527752519.439299
GAGGTTG900.00527752519.439296
AACACTA900.00527752519.439297
GTATAAT2002.4143446E-819.258971
GTATTGG1107.4390887E-419.0998041
AGGGGGG1301.0534747E-418.84942270
ATACTAC950.00721153518.4161683
GCCAATC950.00721153518.41616813
Produced by FastQC (version 0.11.2)