FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2523_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2523_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322096
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9670.3002210521086881No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9660.29991058566390144No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5150.15989021906512346No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT4020.12480751080423229No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATG200.006960267752.47566215
CGTTAAG350.001257286539.981462
AGCGATC604.989948E-429.15314712
TAGGGTG500.007202702628.00006346
TGGCCAT500.00721910627.9870214
TGCGCAT500.00721910627.9870210
GCCTCAC500.00721910627.987027
TAAGATG500.00721910627.987024
CTAATAC700.001227952424.988413
GTATATA1755.2077667E-922.0034681
TATATAA1304.3463097E-621.5284772
TAGTGGT1003.9374622E-420.9902654
GTGTATA850.003781472820.5914781
AGGGGGG1408.187766E-620.00936970
GTTCTAA1055.449036E-420.003151
TAATAGT1408.254014E-619.990734
ATACAAG1055.471905E-419.9907283
GTCTATA900.00526397919.4475081
CAGTACT1107.484459E-419.0820584
TATAAGA1301.0583921E-418.8374182
Produced by FastQC (version 0.11.2)