FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2521_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2521_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349872
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7410.21179174097955825No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7100.20293135775369275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3950.11289843142635021No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATA200.006951157552.49413
TACGCGC200.006951157552.49412
ATCGGCC200.006951157552.49423
GTCCTAT501.7063344E-435.0060041
TAGACCG400.00241849734.9960025
ATAAGTC450.00430681431.1075553
GTATTAG700.001223624325.004291
ATCCGGT851.3074928E-424.7030586
CTTAGCC901.9266355E-423.3306663
GTATTAA952.7728308E-422.1090561
GTATAGG800.00266082321.8787521
GTATACT1304.334939E-621.5364
ATAATAC1003.9291225E-420.9975993
TGCGGAG850.003788081220.58588212
GGTATAC850.003788081220.5858823
CAAGACT850.003788081220.5858824
TAAGCAA850.003788081220.5858825
TACACTG850.003788081220.5858825
ATATAAG1408.212146E-620.0034311
CTCTCGC1055.4603326E-419.9977159
Produced by FastQC (version 0.11.2)