FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2516_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2516_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141351
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3310.2341688421022844No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3240.22921663094000044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1870.13229478390672864No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1850.13087986643179036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTA150.00221854170.018412
ATCCTAC200.006932270752.5138051
CCTTAGG200.006932270752.5138052
GGATGCT200.006932270752.51380564
CTTACAG200.006932270752.5138053
GACGGGC200.00695170952.47665420
TATAATT450.004290278531.1192934
CAATTAG604.954822E-429.1743371
TATGGGG500.0071800928.0073643
AACACAT850.003768824320.5936495
GCTTGAA1205.660878E-520.42203570
ATTTAAT900.005246454419.4495581
TATGCCG1401.8473838E-417.50460257
GTATGCC1200.001330844217.50460256
CTCGTAT1250.001749206816.80441953
GCCGTCT1300.002273463616.15809460
ATAAAAG1554.0157847E-415.8106073
CGTATGC1350.002924393915.55964755
TGCTTGA1400.003725730815.00394469
CCGTCTT1400.003725730815.00394461
Produced by FastQC (version 0.11.2)