FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2514_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2514_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235919
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3160.13394427748506904No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2910.12334742008909838No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT2680.11359831128480537No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC2630.11147893980561126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG150.00222762569.9615
GCAGCGT200.00695471352.4818731
ACCCACC305.886E-446.6406676
GGTCATG350.001257181539.97771511
AAGGTGC350.001257181539.9777156
GGAGCCC400.002422162834.98058
AGGAGCC400.002422162834.98057
CTAAGCT450.00431333631.0937794
TATACTA450.00431333631.0937795
TTGTCGG500.007181267728.0140951
CCGGGTA500.007196162428.00220726
AGGGGGG1052.0271584E-523.34012470
GGATTAT750.001832411323.3252771
CCAAGAG750.001834673523.3203333
AACAGGT800.002669551221.8628127
TAATATT1003.9356417E-420.98834
GTATACT850.003794201920.5767634
AAGGGGG1205.680297E-520.42260669
GGTTCTC900.00528167519.4336138
TTGCGTA1451.1122982E-519.30777217
Produced by FastQC (version 0.11.2)