FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2512_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2512_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162395
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1710.10529880846085163No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC150.002226345769.96127
CTATAGG200.006948106552.487062
GACTAAA200.006956578752.470911
AACACCC305.8804645E-446.6407977
AATAGCG350.001256006839.9778257
GTATTAC556.6609045E-638.1841741
GGTTATA501.7034121E-435.0021551
CACCCAA400.002419912334.98069
GTTATAA552.9811385E-431.8201451
CGATGCT450.00428989431.1226232
AGGGGGG802.4502242E-630.62688870
GTGTTAA604.9638806E-429.1684631
CGGTGTG500.00719025428.00172434
GTTAGAA500.007201083527.99312
TATTACA755.568329E-527.9930972
ACACCCA500.00721192627.9844788
TTAAACA700.001223777724.9938374
ATTAAAC851.3044455E-424.6997953
GAGCAAC851.3071785E-424.69218811
ACAATAC750.001828909323.3275813
Produced by FastQC (version 0.11.2)