FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2511_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2511_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259763
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6060.23328957549766516No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5190.1997975077281984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2730.10509579886280955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGC200.00694623152.49956525
GAGCGCC200.006972738552.4490369
AGGCGTA400.002409247735.01995536
TAGGCGT552.977143E-431.8363235
TATCGTT657.969905E-426.90735
AGGCATA1202.154733E-623.33763748
GTATAAG952.7807718E-422.0965631
TAACAGT800.002652844421.8874766
TATTAGA1205.721638E-520.4047052
GGCATAG1257.785556E-519.60361549
CGTGAAG900.0052957419.4255714
GTGTCGG900.0052957419.425579
ACCATCG1107.504703E-419.0723788
AGAGTTC1107.504703E-419.0723788
GATTATA1301.05681844E-418.8387371
TAGGCAT1501.4893085E-518.66651347
GGATAAC950.007212303618.4138031
CATATAA950.007220324618.410262
TTAGGCA1150.001005213118.26071746
CAATGCG1000.00963907517.50997751
Produced by FastQC (version 0.11.2)