FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2501_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2501_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183503
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2300.12533855032342797No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC1890.10299559135272993No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC200.006954975452.47673
ACCTTAC200.006954975452.47677
ACTACAC350.001255751139.9822463
TCCCGAG400.002419419134.98446710
TAAGCCA552.9867276E-431.81272946
GAGTGGT500.00721044227.9875747
CTAAGAG500.00721044227.9875743
TACATCG500.00721044227.9875745
TTCACAG657.9290255E-426.92580430
TAGGACA750.001831918123.3229794
GAATAAG800.00265292521.883181
TAATTGT800.002665557421.8652924
TTTATAC800.002665557421.8652923
AGGGGGG1150.00100110918.26769870
CCTTTAA1000.00963949117.5065441
CAAAGCC1000.00965464417.50177257
ATTATAT1200.001339233517.4922353
AGGAGGG1000.00968500817.4922337
TACATAC1000.00968500817.4922332
ACAGATT1000.00968500817.4922338
Produced by FastQC (version 0.11.2)