FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2499_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2499_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550070
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15950.2899630956060138No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG13840.2516043412656571No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9300.16906939116839675No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5940.1079862562946534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGA200.0069717952.45868311
CGCGCGG200.0069717952.45868310
ACCTTAG703.497665E-529.9872951
AGGGGGG3500.026.01027570
AAGGGGG3950.024.81993369
CCTATAC851.3131936E-424.690933
CACCGGG750.001829549223.33830631
CCTACAC800.002674779921.861763
TCTATAC1304.3609252E-621.5254273
GGGGGTA850.003779621820.59637339
GTCCTAT850.003797594520.5795151
CAGTGCA1205.7454577E-520.40069
GTATAGG900.00528636319.436211
TATAGGG900.00529192419.4326762
AGAGCGC1451.1230399E-519.2951498
CTACACT1301.06127954E-418.8347474
ATAGTGC950.00723874218.4065558
GTCTTAA1150.001009975718.2531381
GCATAGG2952.1827873E-1117.80526550
GGCATAG3153.6379788E-1217.78480549
Produced by FastQC (version 0.11.2)