FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2498_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2498_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495345
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7940.16029232151328873No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7730.1560528520526098No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT450.00430328931.11528628
TCTACGG700.001228220524.9907322
TATTAGG1102.9274945E-522.2644712
GTATAGG800.002667636821.8713071
ACAGTCC800.002670766621.8668928
GTATCAG1003.9337508E-420.9964561
AAGGGGG3100.019.19612569
AGGGGGG2951.8189894E-1218.98559870
GTGTTAA950.00721197118.4179441
TACTATA950.00722037918.4142252
TTAATAC1351.4133041E-418.1432533
GTATTGG1200.001341123817.4970461
TATAGGA1000.00970350517.4935132
TACAGTG1200.001342940617.4935137
TGCTTGA3700.017.03275355
GCCGTCT3359.094947E-1216.71359646
CTGCTTG3651.8189894E-1216.30520254
TAGGACA2602.7623173E-816.1494884
GTGTAGT1554.0545381E-415.8037831
GCTTGAA4050.015.5623656
Produced by FastQC (version 0.11.2)