FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2497_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2497_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476001
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15490.32541948441284785No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG13580.28529351829092797No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT8620.181092056529293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4790.10063004069319181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2400.032.09253770
CTAACCT700.001228709524.9887814
TAAGACC800.002671826921.8651814
GAACTTA1003.9408854E-420.9905766
GTCATAG850.003792789520.583321
GCTATAA850.003792789520.583321
AAGGGGG3750.020.53922569
TATTAAG900.005286110619.4357172
GTATATC1107.4803183E-419.0863531
GATCTAC1301.05791696E-418.8416541
ACCTATA1501.4870784E-518.67595163
AGACCTT1150.001009875418.2526746
GTATGGA1200.001341648217.4958211
GTATACA1602.6075566E-517.4958211
TCCTATA1000.0097073317.4921462
CATAATA2401.0308213E-817.4921462
CTAGAGG2259.41327E-817.1034323
GTATTAT1906.1204937E-616.574991
TATACAG1704.3940538E-516.4631965
GTATATA2401.9585787E-716.0378361
Produced by FastQC (version 0.11.2)