FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2496_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2496_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482528
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC25420.5268088069500629No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG17460.36184428675641617No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACCG450.004308942231.106789
GCCTGGA450.004308942231.1067828
TAGGTCG500.00720753727.9990065
GCATAGC808.6564316E-526.25178756
GTGTCCC800.002667080621.8719569
GTATTGG1355.9984086E-620.7464541
TCTGGAC1205.7104502E-520.4159413
CATAGGC2603.6379788E-1220.19159151
CTGGACA1055.461554E-419.999294
TAGTATC1055.4653705E-419.9972177
CCAACCC900.00527359919.4437544
GCATAGG2705.456968E-1219.44375250
CGACCTC1651.589784E-619.090236
TATGATC1107.4703095E-419.090234
TCGACCT1651.589784E-619.090235
ATATACT2053.2314347E-818.7798214
TACCCTT950.00720616718.4203995
ACTGTCC950.007210477318.418498
ATAACGT950.007210477318.4184925
ATCGAGG950.007210477318.4184929
Produced by FastQC (version 0.11.2)