FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2495_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2495_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405783
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC26270.6473903539576571No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG19720.4859740304547011No Hit
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT9320.22967940993092367No Hit
GGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAA7070.1742310545291449No Hit
GTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAA6940.17102737177259766No Hit
AATAAAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGA4370.10769302804701035No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCATAGG4300.10596796810117724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT459.1818314E-538.89574438
GCTTGCG501.7080054E-435.0018542
GCAGCGT601.2172506E-534.9889071
CTTGCGT552.9934655E-431.81201743
TCGTTAC450.0043118931.10125224
ACTGCTA450.00431709531.0935848
CGCGACC450.00431709531.09358412
CTCACGT500.00719876128.00493228
TACGCTT657.942227E-426.9278239
TGTACGC657.942227E-426.9278237
CGCTTGC657.942227E-426.9278241
ACGCTTG700.001223928325.00440440
GCACAAC750.001825185323.3460856
CGACTGC750.001837008723.3201896
TAGGCGT952.7737996E-422.10915835
GTTATAA1304.3453856E-621.5316351
AGCACAT1003.9425545E-420.98816910
ATTACAC1003.9425545E-420.9881693
TCGACCT1356.038221E-620.7290575
TTAGGCG850.00377733120.59694334