FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2491_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2491_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311138
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6700.2153385314554956No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6660.214052928282627No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT5000.16070039660857882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3710.11923969428356548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG200.006963342452.469381
GCTGGTC500.007228625527.9791728
TATAGTG500.007228625527.9791725
ACTCCGT500.007228625527.9791726
CTTAGCA500.007228625527.9791722
TTAGGCG808.628295E-526.26001728
TAGAACC959.423382E-625.7702884
AAGGGGG2250.024.90239369
GTTCTAA750.00183626223.3197251
CATGAAC750.001837980423.3159773
GTATTAA800.002671852221.8622421
GGCTAGA800.002671852221.8622421
GCATAGG2259.094947E-1221.78608950
TAACACA1304.3561013E-621.5224384
GTACTTC1003.9445097E-420.9843796
AGGGGGG2700.020.74531770
AAGAGTG1205.7332843E-520.401485
GTATTAT1055.475746E-419.9883331
CTAGAAC1257.847717E-519.5854193
TATTAGA1257.847717E-519.5854192
Produced by FastQC (version 0.11.2)