FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2487_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2487_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146022
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6780.4643135965813371No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6620.45335634356466836No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG252.3923065E-455.9610941
GTTACGA200.006959046752.4635289
TTACGTA200.006959046752.4635284
AAGGGCT350.001250119340.0133227
GCCATGG350.00125645839.9722141
TCTTAAT400.002420783734.9756852
GTATTTA450.00431090731.0894971
TTAGGCA959.300566E-625.79806340
TAAGCCA750.001825511223.33310546
TTATTAT750.001832807923.31712210
ATAGGCA1154.0774103E-521.31144158
GTTCTCC950.00721008818.4082551
AGGCATA1906.0293078E-616.58446954
GGCATAG1755.551263E-516.0053349
GGCGAAT2653.408786E-815.85433570
CATAGGC1554.0252603E-415.80629745
TAGGCAT1858.923132E-515.14017753
TATTCTG1400.00375035514.989585
GCATAGG1901.11672685E-414.74680662
AAAGCCA1500.005876103414.00946464
Produced by FastQC (version 0.11.2)