FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2484_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2484_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369145
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC13400.36300098877135No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG8920.24163946416719717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4150.11242194801500764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAG400.002422851734.9836732
CCTGGAC450.004314548331.09663
GTATTAA500.00721577827.9907321
GGGGAGC500.00722055327.9869386
GTGTAAA851.3096078E-424.6977041
TAGAGTA750.001825552623.3445962
GTATAGA1052.0409872E-523.325611
ATACTAT901.9315192E-423.3224496
CGGTGTG1253.0951142E-622.3895515
TAAACTA1957.7852746E-1021.5284165
CTCCTAC850.003784301520.5897936
ATAGGCT1257.7872086E-519.60679670
AGGGGGG1257.7872086E-519.60679670
GGCATAG2551.1114025E-917.85174655
GCATAGG2401.0168151E-817.50844456
ACACCAG1000.00965897217.50606731
GTGTAAT1000.00969674117.4942071
GTCCAGA1000.00969674117.4942071
GAGGTAT1000.009704309517.4918376
AGGTGAC1200.001344143517.489477
Produced by FastQC (version 0.11.2)