FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2480_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2480_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185352
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4420.23846519055634685No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4310.23253053649272734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140.11545599723768829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC150.0022261769.966534
TCCGTGC200.006941180752.50324226
ACCGTCC400.002413481735.0021647
CGCACCG400.002413481735.0021644
TACACAG501.7097608E-434.9832655
ACAGTGC652.109202E-532.2748228
CAGTGCA652.109202E-532.2748229
CAGCACG552.9827532E-431.82014834
ACCGCAT450.00429224331.12143566
AACGCAC450.00429792331.11303142
TAGCTGC450.00429792331.11303124
ACAGCAC604.9665564E-429.16846733
ACGCACC500.007192833328.00172843
TTAGGCA901.9160667E-423.34107852
CCACGCA750.001826600223.33477425
GTGCTTT750.001832350423.3221766
TATACTG952.7781966E-422.0946945
ACCACGC800.002657834221.8763524
TCATACA800.002666183721.8645422
CACAGTG800.002674554721.8527457
Produced by FastQC (version 0.11.2)