FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2478_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2478_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110060
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2500.22714882791204796No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC2250.20443394512084317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1480.1344721061239324No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT1360.1235689623841541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACA150.002220860469.989544
GATTACG200.00692704452.5160337
TATCGGT200.00695200852.46830710
ATAATGT400.002411733634.9947743
TATACTC552.9786234E-431.8134295
ATATGCT450.004294845331.1064664
GTTAATA450.004294845331.1064661
TCACTGA450.004294845331.10646644
ACAACTT604.9597316E-429.1623085
GCATAGG700.001217343125.00763356
GAATATA901.9154283E-423.329851
TATAATG750.001824147423.329852
GGCATAG800.00264728921.8816855
TGATAGT800.002654293321.8717334
TCCTGAA850.003762663820.59452248
AGGCATA950.00715761418.42667854
ATATAAT1351.399394E-418.1454371
TAGGCAT1000.00961947117.50534453
CCAAAAA2000.002766472312.24260713
AGGCGAA2601.1545582E-412.11908370
Produced by FastQC (version 0.11.2)