FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_qg2477_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_qg2477_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188390
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4600.2441743192313817No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4090.2171028186209459No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1970.10457030627952651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG400.002416295734.994161
AACATCG450.004302921231.105925
CTGCACT604.97353E-429.1617989
CTAGACT755.569988E-527.9953294
ATAATCC500.007201174327.9953293
CTAGTCG500.007201174327.99532915
TATACTT657.942257E-426.9185835
TTATACT808.642763E-526.2456194
ACTGATC700.001223939724.9958298
GAGTTGA1000.00967049717.49708211
CCTAGTG2552.1471351E-816.467843
TCATAAA1300.002283799716.1511512
CCCTAGT2653.4482582E-815.8464112
CCCCTAG2704.3391992E-815.5529591
AGGCGAA4700.014.89508670
CTCTAGA1656.492583E-414.8460082
TAGTGAC3001.5773549E-713.9976645
TCTAGAC1600.00906916113.1228093
GCGAATC2951.9906038E-613.052134570
AAGGCGA5500.012.72852769
Produced by FastQC (version 0.11.2)